nebnext multiplex oligos for illumina (New England Biolabs)
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New England Biolabs
nebnext multiplex oligos for illumina
Nebnext Multiplex Oligos For Illumina, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 527 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/module/NEBNext+Ultra+II+Ligation+Module/pmc13651819-675-34-34
Average 97 stars, based on 527 article reviews
Nebnext Multiplex Oligos For Illumina, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 527 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/module/NEBNext+Ultra+II+Ligation+Module/pmc13651819-675-34-34
Average 97 stars, based on 527 article reviews
nebnext multiplex oligos for illumina - by Bioz Stars,
2026-10
97/100 stars
Images
1) Product Images from "Gapmers targeting conserved SARS-CoV-2 single-stranded regions reduce viral load and reshape inflammatory macrophage responses"
Article Title: Gapmers targeting conserved SARS-CoV-2 single-stranded regions reduce viral load and reshape inflammatory macrophage responses
Journal: iScience
doi: 10.1016/j.isci.2026.117639
Figure Legend Snippet:
Techniques Used: Virus, Variant Assay, Recombinant, Reverse Transcription, Diagnostic Assay, Luminex, Ligation, Multiplex Assay, Generated, Software
Related Articles
Ligation:Article Title: High-throughput evaluation of in vitro CRISPR activities enables optimized large-scale multiplex enrichment of rare variants. Article Snippet: Previous high-throughput evaluations of CRISPR activities for a large number of target and guide RNA sequences were based on measuring insertion– deletion frequencies rather than cleavage efficiencies.. Here we develop two high-throughput in vitro methods, Cut-seq1 and Cut-seq2, to evaluate Cas9 cleavage efficiency for tens of thousands, or even hundreds of thousands, of guide RNA–target pairs.. These methods reveal low correlations between in vitro cleavage efficiencies and insertion–deletion frequencies in cells, yet high concordances in protospacer adjacent motif compatibility. Article Title: NAP-seq for full-length noncapped RNA sequencing. Article Snippet: The majority of the mammalian genome is transcribed into RNAs, most of which are noncapped RNAs (napRNAs) that not only regulate diverse biological processes through their functions as noncoding RNAs but also serve as processing products to delineate specific RNA biogenesis pathways.. However, due to their heterogeneous lengths, diverse terminal modifications and complex secondary structures, identifying these napRNAs poses substantial challenges.. Recently, we developed a napRNA sequencing technique (NAP-seq) to identify full-length sequences of napRNAs with various terminal modifications at single-nucleotide resolution. Formalin-fixed Paraffin-Embedded:Article Title: Improved deconvolution of circulating tumor DNA from ultra-low-pass whole-genome methylation sequencing using CelFiE-ISH Article Snippet: .. End repair and A-tailing used NEBNext ® FFPE DNA Repair Mix (NEB #M6630) and NEBNext ® UltraTM II End Repair/dA-Tailing Incubation:Article Title: Improved deconvolution of circulating tumor DNA from ultra-low-pass whole-genome methylation sequencing using CelFiE-ISH Article Snippet: .. End repair and A-tailing used NEBNext ® FFPE DNA Repair Mix (NEB #M6630) and NEBNext ® UltraTM II End Repair/dA-Tailing Purification:Article Title: Molecular Epidemiological Investigation of Salmonid Alphavirus Genotype 2 (SAV2) Emergence on the Helgeland Coast of Norway in 2023 Article Snippet: Sequencing libraries were prepared using Oxford Nanopore Technologies (ONT, Oxford, UK) ligation-based protocols. .. Purified amplicon pools (400 ng) were end-repaired and dA-tailed using the NEBNext Ultra II End Repair/dA-Tailing Amplification:Article Title: Molecular Epidemiological Investigation of Salmonid Alphavirus Genotype 2 (SAV2) Emergence on the Helgeland Coast of Norway in 2023 Article Snippet: Sequencing libraries were prepared using Oxford Nanopore Technologies (ONT, Oxford, UK) ligation-based protocols. .. Purified amplicon pools (400 ng) were end-repaired and dA-tailed using the NEBNext Ultra II End Repair/dA-Tailing Article Title: Targeted long-read RNA sequencing for rare disease diagnosis and variant interpretation Article Snippet: Captured cDNA was amplified using on-bead PCR with KAPA HiFi HotStart ReadyMix under the following program: initial denaturation at 95°C for 3 min, 14 cycles of (98°C for 20 s, 67°C for 20 s, and 72°C for 5 min), and final extension at 72°C for 8 min. PCR products were purified with 0.7X SPRIselect beads and quantified using the Qubit dsDNA High Sensitivity Assay and Agilent High Sensitivity D5000 ScreenTape Assay on an Agilent 4200 TapeStation. .. Pre- and postcapture amplified cDNA products were end-repaired and dA-tailed using the NEBNext Ultra II End Repair/dA-Tailing Binding Assay:Article Title: NAP-seq for full-length noncapped RNA sequencing. Article Snippet: The majority of the mammalian genome is transcribed into RNAs, most of which are noncapped RNAs (napRNAs) that not only regulate diverse biological processes through their functions as noncoding RNAs but also serve as processing products to delineate specific RNA biogenesis pathways.. However, due to their heterogeneous lengths, diverse terminal modifications and complex secondary structures, identifying these napRNAs poses substantial challenges.. Recently, we developed a napRNA sequencing technique (NAP-seq) to identify full-length sequences of napRNAs with various terminal modifications at single-nucleotide resolution. Reverse Transcription:Article Title: NAP-seq for full-length noncapped RNA sequencing. Article Snippet: The majority of the mammalian genome is transcribed into RNAs, most of which are noncapped RNAs (napRNAs) that not only regulate diverse biological processes through their functions as noncoding RNAs but also serve as processing products to delineate specific RNA biogenesis pathways.. However, due to their heterogeneous lengths, diverse terminal modifications and complex secondary structures, identifying these napRNAs poses substantial challenges.. Recently, we developed a napRNA sequencing technique (NAP-seq) to identify full-length sequences of napRNAs with various terminal modifications at single-nucleotide resolution. |
